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ãáÎÕ ÇáÈÍË
Êã ÊãíÒ ÓÈÚ ãÚÒæáÇÇÊ ãä ÇáãÝØ æå Ñ ÇáãäÝØÑå ÊÍÊ äæÚ ÇáãäÝØÑå ÚÒáÊ ãä æáÇíÇÊ ÇáÌÒíÑå¡ ÇáÎÑØæã¡ Çáäíá ÇáÇÈíÖ¡
ÇáãÚ Òæáæ ãä Êä Ç ÒäíÇ áããÞÇÑäæ ÈÇÓÊÎÏÇã )T1/ ÔãÇá ßÑÏÝÇä¡ ¡ÔãÇá ÏÇÑÝæÑ æ ÇáÇÓÊæÇÆíæ( ÇÓÊÎÏãÊ ÇáÚÊÑå ÇáãÑÌÚíæ ) 44
ÐÇÊ ÇáãÓÊÚã Ç ÑÊ (Mycoplasma subsp mycoides subsp mycoides ÈÇÏÆííä ÇÍÏìãÇ ÎÇÕ ÈÇáãÝØæÑå äæíÚ ÇáãäÝØÑå
äÊÌ Úä ÇáÈÇÏÆæ ÇáãÎÕÕæ ÞØÚ ØæáíÇ .(M.mycoides cluster) æÇáÇÎ Ñì áÍÒãÉ ÇáãÝØæÑå ÇáãÊÝØÑå (Mmm sc) ÇáÕÛíÑå
ÊÞÑíÈÇ 572 ÒæÌ ÞÇÚÏì ¡ ÇãÇ ÇáÈÇÏÆæ ÇáÇÎ Ñì ÝÞÏ äÊÌ ÚäíÇ ÞØÚ ØæáíÇ 064 ÒæÌ ÞÇÚÏì. æáÊÃßíÏ ìæíæ ìÐå ÇáÚÊ Ç ÑÊ ÇÌÑí
ÍíË äÊÌ Úä Ðáß ÞØÚÊÇä ÇØæÇáíãÇ 04 æ 545 ÒæÌ ÞÇÚÏí ããÇ íæßÏ Çä ÌãíÚ Ase ÇÎÊÈÇÑ ÇáíÖã ÇáÎãÇÆÑì ÇáãÞíÏ áÎãíÑÉ 1
ÇáãÚÒæáÇÊ ÌäÓ ÇáãÝØæÑå ÇáãÊÝØÑå ä æíÚ ÇáãäÝØÑå ÇáãÓÓÈÈæ áãÑÖ ÐÇÊ ÇáÑÆæ ÇáÈãæÑì ÇáÓÇÑì Ýì ÇáÇÈÞÇÑ æÊÔÇÈæ ìæíÉ ÌãíÚ
æÇäíÇ ÊÚØí ÇØæÇá ãÊÓÇæíæ ÚäÏ ÇÌ Ç ÑÁ ÇÎÊÈÇÑ ÇáÞØÚ ÇáÎãÇÆÑí ÚãííÇ. Mmm sc ÇáãÚÒæáÇÊ ãä äæÚ ÇáãÝØæÑå ÇáãÊÝØÑå
Summary
Seven isolates of Mycoplasma mycoides subsp. mycoides Sc. Mmm sc (GZ,
KH1, KH2, WN, B, F and KH3J) were isolated and the Tanzanian reference
strain were subjected to Polymerase Chain Reaction (PCR) using low pairs of
oligonucleotid. One primer is specific for M. mycoides subsp. mycoides sctype
(Mmm sc) and the other was for the mycoides cluster (Myc). In all
isolates the pair of Msc primers showed amplifications of 275 bp fragment
following PCR application. The Myc control primers targeted a DNA
sequence of about 460 bp which was used to identify members of the
mycoides clusters including Mmm s.c. The Mmm s.c. specific amplicons were
further subjected to restriction fragment length polymorphism (RFLP) using
Asel restriction enzyme; Bands of 232 and 43 bp were seen. These findings
confirmed identification M. mycoides subsp. Mycoides sc- type strains of the
Sudan and ascertained their affiliation to the Mmm sc. morphotype as based
on PCR and restriction endonuclease cleavage.
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