|
Summary
The genetic diversity and relationships between five Sudanese camels' types;
Anaffi, Arabi, Rashidi, Bishari, and Kenanni were investigated. Genomic DNA was
extracted from 128 camels representing the five types. A polymorphic DNA marker
suitable for genome analysis and population genetic studies was employed. This marker
was Random Amplified Polymorphic DNA (RAPD). RAPD analysis was performed by
PCR with one random primer to amplify the genomic DNA of the different camels' types.
The primer resulted in amplification of 18 polymorphic bands in the five camels' types
that ranged in size from 150 – 1700bp. The mean number of bands that were produced in
the five types ranged from 5.3 to 6.9 in Anaffi and Kenanni types, respectively. The
average value of bands sharing within a type ranged from 0.54 to 0.87 between Kenanni
and Bishari individuals, respectively. The values of average bands sharing between these
five types ranged from 0.212 – 0.359 between Anaffi- Kenanni, and Arabi – Rashidi,
respectively.
The genetic distances estimated from RAPD data showed that the highest distance
value was between Anaffi and Kennani (0.79), and the lowest one was that of Arabi and
Rashidi (0.64).
|