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ãáÎÕ ÇáÈÍË
     Êã ÊÞííã ÇáÇÓÊÌÇÈÉ ÇáãÕáíÉ ááÃÌÓÇã ÇáãÖÇÏÉ (ÇáÖÏ) Ýí ÃÈÞÇÑ ÓæÏÇäíÉ ÐÇÊ ÃÚãÇÑ ãÎÊáÝÉ ¡ äÊíÌÉ áÊÍÕíäåÇ ÈãÓÊÍÖÑíä ãä áÞÇÍ ÇáÚÊÑÉ 19 ááÈÑæÓíáÇ ÇáãÌåÖÉ ÈÇÓÊÎÏÇã ÇÎÊÈÇÑÇÊ ÇáÑæÒÈäÞÇá ÇáÕÍäì ¡ ÊËÈíÊ ÇáãÊããÉ ¡ ÇáÊÑÇÕ ÇáÃäÈæÈí ¡ ÇááÈäí ÇáÍáÞí æ åí ÇÎÊÈÇÑÇÊ ÊÞáíÏíÉ áÊÍÏíÏ ãÓÊæì ÇáÖÏ Ýí ÇáãÕá ¡ ÚáÇæÉ Úáì ÇÎÊÈÇÑí ÇáÇáíÒÇ ÛíÑ ÇáãÈÇÔÑÉ æ ÇáÊäÇÝÓíÉ. ÚäÏ ÇÓÊÎÏÇã ÃÍÏ ÇáãÓÊÍÖÑíä Ýí ÊÍÕíä ãÌãæÚÊíä ãä ÚÌæá ÚãÑåÇ ÚÇãíä ¡ ÇáÃæáì ÈÇáÌÑÚÉ ÇáßÇãáÉ æ ÇáËÇäíÉ ÈÇáÌÑÚÉ ÇáãÎÝÖÉ (10/1 ãä ÇáÌÑÚÉ ÇáßÇãáÉ) ¡ æÌÏ Ãä ÃÚáì ãÊæÓØ ááÖÏ ÈÇÎÊÈÇÑ ÇáÊÑÇÕ åæ 1039.3 æ 256.5  æÍÏÉ ÚÇáãíÉ/ ãá   Úáì ÇáÊæÇáì. áÞÏ ÇäÎÝÖ ãÓÊæì ÇáÖÏ Åáì Ïæä ÇáãÓÊæì ÇáÊÔÎíÕì ÈÇáÇÎÊÈÇÑÇÊ ÇáÊÞáíÏíÉ ÈÚÏ 641 æ 73 íæãÇð ãä ÇáÊÍÕíä ááãÌãæÚÊíä Úáì ÇáÊæÇáì.  ÚäÏ ÊÍÕíä ÈÞÑÊíä (ÚãÑ 10 ÃÚæÇã ææÒä 200 ßíáæ ÌÑÇãð) ÈÇáÌÑÚÉ ÇáãÎÝÖÉ ãä äÝÓ ÇááÞÇÍ æÍÞäåãÇ Ýí äÝÓ ÇáæÞÊ ÈÚÞÇÑ ÇÓÊíæÑíÊ ÇáÏíãíäÇÒíä (ÈÑäíá) ÈáÛ ÃÚáì ãÊæÓØ ááÖÏ ÇáãÊáÒä 307.6 æÍÏÉ ÚÇáãíÉ/ãá æ ÇäÎÝÖ Åáì Ïæä ÇáÍÏ ÇáÊÔÎíÕì ÈÚÏ 35 íæãÇð ãä ÇáÊØÚíã æáßä ÙáÊÇ ãæÌÈÊÇä áÇÎÊÈÇÑ ÊËÈíÊ ÇáãÊããÉÇÍÏÇåãÇ ááíæã 45 æ ÇáÃÎÑì ááíæã 74 áÊÕÈÍ ÓÇáÈÉ Ýí Çáíæã 105 ÈÚÏ ÇáÊÍÕíä. ÚäÏ ÊÍÕíä ÈÞÑÊíä ÇÎÑííä Ýì ÚãÑ 14 ÚÇãÇð ææÒä 300 ßíáæÌÑÇã ÈÇáÌÑÚÉ ÇáãÎÝÖÉ áäÝÓ ÇááÞÇÍ æÌÏ Ãä ÃÚáì ãÊæÓØ ááÖÏ ÇáãÊáÒä 635 æÍÏÉ ÚÇáãíÉ/ãá æ ÇäÎÝÖ Åáì Ïæä ÇáÍÏ ÇáÊÔÎíÕì Ýí Çáíæã 164 ÈÚÏ ÇáÊÍÕíä. ÚäÏ ÇÓÊÚãÇá ÇáãÓÊÍÖÑ ÇáËÇäí áÊÍÕíä ãÌãæÚÊíä ãä ÇáÚÌæá Ýí ÃÚãÇÑ 7-5 æ 14-12 ÔåÑÇð ÈÇáÌÑÚÉ ÇáßÇãáÉ¡ æÌÏ Ãä ÃÚáì ãÊæÓØ ááÖÏ ÇáãÊáÒä ÞÏ ÈáÛ 1108 æ 862 æÍÏÉ ÚÇáãíÉ/ãá ãÕá Úáì ÇáÊæÇáì. æ ÇäÎÝÖ Åáì ÇáãÓÊæì ÇáÓÇáÈ Ýí íæãí 100 æ 85 áÊÍÕíä ÇáãÌãæÚÊíä Úáì ÇáÊæÇáì. ÃãÇ ÈÇáäÓÈÉ áÈÞÑÊíä ÃÎÑííä Ýí ÚãÑ 10ÃÚæÇã æ ÇááÊíä ÍÕäÊÇ ÈÇáÌÑÚÉ ÇáãÎÝÖÉ áåÐÇ ÇááÞÇÍ ÇáËÇäí ÃíÖÇ ¡ ÝÞÏ ÈáÛ ÃÚáì ãÊæÓØ ááÖÏ ÇáãÊáÒä 1723.5 æÍÏÉ ÚÇáãíÉ/ãá ¡ æ ÇäÎÝÖ Åáì Ïæä ÇáÍÏ ÇáÊÔÎíÕì Ýí Çáíæã 105 ÈÚÏ ÇáÊÍÕíä. ÙáÊ ÅÍÏì ÇáÈÞÑÊíä ãæÌÈÉ áÇÎÊÈÇÑ ÇááÈä ÇáÍáÞí ÍÊì Çáíæã 31 ÈÚÏ ÇáÊÍÕíä. æÌÏ ÈÚÏ ãÖì 960 íæãÇð ãä ÇáÊÍÕíä Ãä 4 ãä ÚÌæá ÍÕäÊ ÈÇáÌÑÚÉ ÇáßÇãáÉ æ ÇáãÎÝÖÉ æ ÈÞÑÉ ÍÕäÊ ÈÇááÞÇÍ ÇáÃæá ÞÏ ÇÚØÊ äÊíÌÉ ãæÌÈÉ áÇÎÊÈÇÑí ÇáÇáíÒÇ ¡ ÈíäãÇ ÇÙåÑÊ ÅÍÏì ÇáÈÞÑÊíä ãä ÇááÊíä ÍÞäÊÇ ÈÚÞÇÑ ÇáÈÑäíá äÊíÌÉ ÓÇáÈÉ áÇÎÊÈÇÑ ÇáÇáíÒÇ ÇáÊäÇÝÓíÉ. äæÞÔ ÇáÇÎÊáÇÝ Ýí ÇáÇÓÊÌÇÈÇÊ ÇáãÎÊáÝÉ ÇáÓÇÈÞÉ ææÖÚ ãÞÊÑÍ áÊÍÕíä ÇáÃÈÞÇÑ ÇáÓæÏÇäíÉ ÈÃÚãÇÑåÇ ÇáãÎÊáÝÉ ÈæÇÓØÉ áÞÇÍ ÇáÚÊÑÉ 19 ááÈÑæÓíáÇ ÇáãÌåÖÉ ÊÍÊ ÙÑæÝ ÇáÑÚì ÇáÊÞáíÏíÉ .


Summary
Serum antibody responses of Sudanese cattle of different age groups were assessed following their immunization with two B. abortus strain 19 vaccines. The conventional serological tests (RBPT, CFT, SAT, and MRT) for brucellosis, indirect and competitive ELISAs (iELISA and cELISA) were used. Using the first vaccine, the highest mean SAT antibody levels of two years old calves immunized with standard and reduced doses of the vaccine were 1039.3 i.u/ml and 256.5 i.u/ml, respectively. They reacted below diagnostic levels to the conventional
                                                  

tests  on  day  164  and  73 p.v., respectively.  The highest mean SAT
antibody level of 10 years old and 200kg body weight cows vaccinated
with the reduced dose of the vaccine and inoculated simultaneously with diminazene aceturate was 307.6 i.u/ml, which receded to negative status on day 35. These two cows were both positive for CFT until day 45 and one of them on day 73; the latter became negative on day 105. The highest mean SAT antibody level of 14 years 300 kg body weight cows immunized with the reduced dose was 635.6 i.u/ml. They reacted below diagnostic levels to the conventional tests on day 164. Using the second vaccine, the highest mean SAT antibody levels of 5-7 and 12-14 months old calves that were vaccinated with the full doses were 1108 and 862 i.u/ml., respectively.  These titres receded to negative status on days 100 and 85, respectively. The highest mean SAT antibody level of cows over 10 years old, which were vaccinated with reduced doses of the vaccine, was 1723.05 i.u/ml.; this titre receded to a negative value on day 105. One cow of the latter group was positive for MRT till day 31.  Variations in response to vaccination are discussed. On day 960 p.v. four calves vaccinated at two years of age with full or reduced doses and one cow vaccinated with a reduced dose of the first vaccine were positive to the iELISA and cELISA, while the other vaccinated cow which was injected with the diminazene aceturate was negative to cELISA. Suggestions are made for a protocol of vaccination of different age groups of Sudanese cattle, under traditional farming system, with S19 vaccine.

 
 
   
 
 

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