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ãáÎÕ ÇáÈÍË
ÇÓÊÎÏãÊ ÈÑæÊíäÇÊ ÌÓÏ ÇáãÊæÑÞÉ ÇáÚãáÇÞÉ (Fasciola gigantica) æãäÊÌÇÊåÇ ÇáÅÎÑÇÌíÉ æÇáÅÝÑÇÒíÉ ÈÚÏ ÍÖÇäÊåÇ Ýí æÓØ ÛÐÇÆí (RPMI-1640). ÍááÊ åÐå ÇáÈÑæÊíäÇÊ ÈæÇÓØÉ ÊÞäíÉ ÇáÑÍáÇä ÇáßåÑÈÇÆí áÝÕá ãßæäÇÊåÇ ÇáãÎÊáÝÉ ÍÓÈ ÃæÒÇäåÇ ÇáÌÒÆíÉ æÐáß áÅÌÑÇÁ ãÒíÏ ãä ÇáÏÑÇÓÉ áãÚÑÝÉ ÇáÈÑæÊíäÇÊ ÐÇÊ ÇáÊÝÇÚá ÇáãäÇÚí æÇáÊí íãßä Çä ÊÓÊÎÏã Ýí ÇáÊÔÎíÕ ÇáãÈßÑ áÍÏæË ÇáÇÕÇÈÉ ÈåÐå ÇáÏíÏÇä æÅíÌÇÏ áÞÇÍ ÖÏåÇ.
ÃÙåÑÊ ÊÞäíÉ ÇáÑÍáÇä ÇáßåÑÈÇÆí Ãä ÌÓÏ ÇáØÝíáí æãäÊÌÇÊå ÇáÅÎÑÇÌíÉ æÇáÅÝÑÇÒíÉ ÊÍÊæíÇä Úáí ãÌãæÚÉ ãä ÇáÈÑæÊíäÇÊ ÐÇÊ ÇáÃæÒÇä ÇáÌÒíÆíÉ ÇáãÎÊáÝå. ææÌÏ  Ãä ÚÏÏ ÇáÈÑæÊíäÇÊ ÇáãÓÊÎÏãÉ ãä ãäÊÌÇÊ ÌÓÏ ÇáØÝíáí ÇáÅÎÑÇÌíÉ æÇáÅÝÑÇÒíÉ ÃÞá ãä Êáß ÇáãÓÊÎáÕÉ ãä ÌÓÏå.  ÊÌãÚÊ ÇáÍÒã ÇáÈÑæÊíäíÉ ÇáÃßËÑ æÌæÏÇð æÇáãÓÊÎáÕÉ ãä ãäÊÌÇÊ ÇáØÝíáí Ýí ÇáÇæÒÇä ÇáÌÒíÆíÉ11 ¡27 æ30 ßíáæ ÏÇáÊæä. æÌÏäÇ ÇáÍÒã ÇáÈÑæÊíäíÉ ÇáãåãÉ ÐÇÊ ÇáÇæÒÇä  44¡ 66¡ ßíáæ ÏÇáÊæä Ýí ãäÊÌÇÊ ÇáØÝíáí.
ÊÍÊæí ÇáÍÒã ÇáÈÑæÊíäíÉ ÐÇÊ ÇáÇæÒÇä ÇáÌÒíÆíÉ 11.5¡æ30-27 ßíáæ ÏÇáÊæä Úáí ÇáÎãÇÆÑ (ÇáÅäÒíãÇÊ) ÇáÑÆíÓÉ æÇáÊí ÊáÚÈ ÏæÑÇð ãåãÇð Ýí ÇáæÙÇÆÝ ÇáÅÍíÇÆíÉ æÇáÊäÎæÑ ÇáãäÇÚí ááØæÑíä ÇáíÇÝÚ æÇáÈÇáÛ ááØÝíáí ÅÐ ÊÍÊæì Úáí ÎãÇÆÑ ÚÇÆáÉ ÈÑæÊíäÇÊ ÔÈíå ÇáÓÇíæäíä (FSAP2) æÈÑæÊäíäÒÇÊ ÇáÓíÓÊíä æÊÑÇäÓÝíÑíÒ ÇáÌáæÊÇËæíä. ÅÓ æÇáåíãæÌáæÈíä æÊÚÊÈÑ åÐå ÇáÈÑæÊíäÇÊ ÇáÂä ãä ÇáÎãÇÆÑ ÇáãÑÔÍÉ ááÇÓÊÎÏÇã ßáÞÇÍ ááÓíØÑÉ Úáí ÇáãÑÖ.

 

Summary
        Fasciola gigantica proteins were extracted from its somatic (SO) and excretory–secretary (E/S) products and analyzed for studying their protein structures that could further be used for detection of its immunoreactive proteins. These proteins can be used for early diagnosis of the disease and development of a protective vaccine. Separation of both parasite products by SDS-PAGE resulted in protein bands of different molecular weights. The execretory/secretory products (E/S) contained fewer bands compared to the somatic extracts (SO). Dominant bands for both SO and E/S products were clustered between 11, 27 and 30 Kda. Major bands of 44, 55 and 66 Kda were observed in E/S products.
The protein bands clustered between 11, 27 and 30 Kda contain the major enzymes that could play important biological and immune-modulatory functions in juvenile and adult parasites such as saponin-like


protein family (FSAP-2), cysteine proteinases, glutathione-S-transferase and haemoglobinase. These proteins can be considered as possible vaccine candidates for the disease control.

 

 
 
   
 
 

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