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Summary
An experiment was conducted to assess the re-isolation and antibody detection of Mycoplasma iowae (Mi) serovar I. Specific-pathogen-free (SPF) turkey hens were inoculated via the oviduct. Post-infection recovery of Mi serovar I was obtained from oviduct and cloacal swabs but not from tracheal swabs. A recovery of 87.5% was revealed by direct culturing onto mycoplasma agar while 78.2% recovery was obtained by indirect culturing through an overlay medium. Although swabbing and culturing was continued for six weeks, isolation of Mi was encountered only in the first three weeks. Anti Mi antibodies were detected by Enzyme Linked Immunosorbent Assay (ELISA) test but not by the Rapid Serum Agglutination( RSAT) test .

 

 
 
   
 
 

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