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ãáÎÕ ÇáÈÍË
         ÃÓÊÎáÕÊ ãÓÊÖÏÇÊ ãä ÌÓÏ ØÝíá ÇáãÊæÑÞÉ ÇáÚãáÇÞÉ (Fasciola gigantica) æãäÊÌÇÊåÇ ÇáÅÎÑÇÌíå æÇáÅÝÑÇÒíÉ. ÍíË Êã ÊÍáíáåÇ áãÚÑÝÉ ÇáÈÑæÊíäÇÊ ÇáããäÚå æ ÇáÊì íãßä ÅÓÊÎÏÇãåÇ Ýì ÇáÊÔÎíÕ ÇáãÈßÑ æ ÇáÊÍÕíä ÖÏ ãÑÖ ÇÈæßÈíÏå. ÃÓÝÑÊ ÊÞäíÉ ÇáÑÍáÇä ÇáßåÑÈÇÆí  (SDS –PAGE) Úä æÌæÏ ÚÏÉ ÍÒã ÈÑæÊíäíÉ ÐÇÊ ÃæÒÇä ÌÒíÆíÉ ãÎÊáÝÉ Ýí ßá ãä ÌÓÏ ÇáØÝíá æãäÊÌÇÊåÇ ÇáÅÎÑÇÌíå æÇáÅÝÑÇÒíÉ ÇáãÎÊáÝÉ.
ÚäÏ ÇáßÔÝ Úä ÇáÍÒã ÇáÈÑæÊíäíÉ ÈæÇÓØÉ ÇáÇãÕÇá ÇáããäÚÉ ØÈíÚíÇð æ ãÚãáíÇ ÈÇÓÊÎÏÇã ÊÞÇäÉ ÇáÊÎØíØ ÇáÛÑÈí (Western blotting)  Êã ÇáÊÚÑÝ Úáí ÚÏÏ ãä ÇáÍÒã ÇáÈÑæÊíäíÉ ÇæÒÇäåÇ ÇáÌÒíÆíÉ  ÊÑÇæÍÊ Èíä27-30 ßíáæ ÏÇáÊæä. ßãÇ ÃíÖÇ Êã ÈæÇÓØÉ ÇãÕÇá ÍíæÇäÇÊ ãÕÇÈÉ ÈÇáãÊæÑÞÉ ÇáÚãáÇÞÉ ÇáÊÚÑÝ Úáí ÍÒã ÈÑæÊíäíÉ ßÇäÊ ÇæÒÇäåÇ ÇáÌÒíÆíÉ 44, 50, 60, 100æ120 ßíáæ ÏÇáÊæä ãä ÇáãääÊÌÇÊ ÇáÅÎÑÇÌíå æ ÇáÃÝÑÇÒíÉ áÝÊÑÉ 5-8 ÃÓÇÈíÚ. ÎáÕ ÇáÈÍË Çáí Ãä ÇáÍÒã ÇáÈÑæÊíäíå ÐÇÊ ÇáÃæÒÇä ÇáÌÒíÆíå  27-30 ßíáæ ÏÇáÊæä ÊÍÊæí Úáí ÇáÅäÒíãÇÊ ÇáÊí íãßä Çä ÊáÚÈ ÏæÑ ÑÆíÓ ááäÔÇØ ÇáÍíæì æÇáãÍÝÒ ááÌåÇÒ ÇáãäÇÚí æ ÇáÊí íãßä ÊÌÑÈÊåÇ ßáÞÇÍÇÊ  ááÓíØÑå Úáí  ÇáãÑÖ.

Summary
Fasciola gigantica antigens were extracted from the somatic (SO) and excretory –secretary (E/S) products and analyzed for detection of immunoreactive proteins that could be used for early diagnosis and development of a protective vaccine.  SDS-PAGE resulted in protein bands of different molecular weights in both parasite products.
The SO extracts and E/S products of F. gigantica were then probed by Western blotting technique using immune sera from naturally and experimentally infected cattle for identification of immunoreactive antigenic components. Polypeptides between 27 and 30 KDa were identified by the sera of all infected animals in both parasite products.   Proteins of 44, 50, 60, 100 or 120 KDa were also detected in E/S products with 5 –8 weeks post infection antisera.


 

 
 
   
 
 

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