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ãáÎÕ áÈÍË

Êã  ÅÎÊÈÇÑ ãÇÆÉ ÚíäÉ ãÕáíÉ ãä ÇáÃÈÞÇÑ ÇáÓæÏÇäíÉ ÈæÇÓØÉ ÇáãÞÇíÓå ÇáãäÇÚíå ÇáãÑÊÈØå ÈÇáÇäÒíã  ÐÇÊ ÇáØæÑ ÇáÓÇÆá æÇáÍÇÕÑÉ   Liquid-Phase Blocking ELISA )) ááÇÖÏÇÏ ÇáäæÚíÉ ááäæÚíä ÇáãÕáííä "O" æ "SAT1" ãä ÝíÑæÓ ÇáÍãì ÇáÞáÇÚíÉ æÐáß ÈÇÓÊÚãÇá ÊÑÇßíÒ ãÎÊáÝÉ ãä ÇáãÓÊÖÏíä "O" æ "SAT1"  æ ãÓÊÍÖÑÇÊ ÇáÇÎÊÈÇÑ ÇáÊì Êã ÊÍÖíÑåÇ Ýí ÇáãÚãá ÇáãÑÌÚí ááÍãì ÇáÞáÇÚíÉ ÈÈÑÈÑÇíÊ   (ÇáããáßÉ ÇáãÊÍÏÉ) .

ÃÙåÑÊ ÇáÏÑÇÓÉ Ãä ÊÑßíÒ äÓÈÉ ÇáãÓÊÖÏ ( ãä ãÓÊÍÖÑÇÊ ÇáÅÎÊÈÇÑ ) Åáí ÇáÃÖÏÇÏ ÇáäæÚíÉ ( Ýí ÇáÚíäÉ ÇáãÕáíÉ) ÅÖÇÝÉ Çáí ÇáÔÑÇåÉ ) (Avidity ÇáãÕáíÉ åãÇ ÃßËÑ ÇáÚæÇãá ÊÃËíÑÇð Úáí ÞÏÑÉ ÇáÊÞäíÉ ÇáãÐßæÑÉ Ýí ÇáßÔÝ Úä ÇáÃÖÏÇÏ ÇáäæÚíÉ Ýí ÇáÚíäÉ ÇáãÕáíÉ. æÌÏÊ Ãä ÃÞá ãÞÏÑÉ áÞíã ÇáÊÞäíÉ ÚäÏãÇ ßÇäÊ äÓÈÉ ÇáãÓÊÖÏ Çáì ÇáÃÖÏÇÏ ÇáäæÚíÉ ãÊÚÇÏáÉ æ ÃÚáì Þíã ÚäÏãÇ ãÇáÊ ÇáäÓÈÉ áÌÇäÈ ÇáãÓÊÖÏ . ßÇäÊ ÇáÚáÇÞÉ ÇáÊáÇÒãíÉ ( Correlation) Èíä ÇáÃãÕÇá ÇáãÑÌÚíÉ ÇáãæÌÈÉ (  ÅÕÇÈÉ ÊÌÑíÈíÉ) æ ÇáÚíäÇÊ ÇáãÕáíÉ ÇáãæÌÈÉ ( ÅÕÇÈÉ ØÈíÚíÉ)  ÇÖÚÝ ãÇ Êßæä ÚäÏ ÅÓÊÚãÇá ÊÑÇßíÒ ãäÎÝÖÉ ãä ÇáãÓÊÖÏ.

            ÅÓÊäÊÌÊ ÇáÏÑÇÓÉ Ãä ÒíÇÏÉ äÓÈÉ ÇáãÓÊÖÏ ÊÒíÏ ãä ãÞÏÑÉ ÇáÊÞäíÉ Úáí ÇáßÔÝ Úä ÇáÃÖÏÇÏ ÇáäæÚíÉ æÐáß áÃä ÇáãÑßÈÇÊ ÇáãäÇÚíÉ ( Immune Complexes)  íãßä ÍÕÑåÇ (blocking) ÈßÝÇÁÉ ÃÚáì Ýí ÍÇáÉ ÒíÇÏÉ äÓÈÉ ÇáãÓÊÖÏ ÃßËÑ ããÇ Ýí ÍÇáÉ ÊÚÇÏá ÇáãÓÊÖÏ æ ÇáÃÖÏÇÏ ÇáäæÚíÉ( Ýí ÇáÚíäÉ ÇáãÕáíÉ) ßãÇ Ãä ÇáÃÖÏÇÏ ÇáäæÚíÉ ÐÇÊ ÇáÃáÝÉ  Affinity)) ÇáÚÇáíÉ Ýí ÇáÃãÕÇá ÖÚíÝÉ ÇáÅíÌÇÈíÉ ÊÊÝÇÚá ÈßÝÇÁÉ ÃßÈÑ ÚäÏ ÒíÇÏÉ äÓÈÉ ÇáãÓÊÖÏ . ÇÓÊäÊÌÊ ÇáÏÑÇÓÉ ÃíÖÇð Ãä ÒíÇÏÉ ÊÑßíÒ ÇáãÓÊÖÏ Åáì ÃÞá ãä ÖÚÝ ÇáÌÑÚÉ ÇáãÞÑÑÉ æ Ðáß Ýí ÍÏæÏ ÇáßËÇÝÉ ÇáÖæÆíÉ ((Optical Density ÇáãØáæÈÉ 1.9 ÊÒíÏ ãä ÍÓÇÓíÉ ÇáÊÞäíÉ Åáí ÏÑÌÇÊ ãÑÛæÈÉ.

Summary

One-hundred Sudanese cattle sera were screened for foot and mouth disease (FMD) serotypes “O” and “SAT1” antibodies by the liquid-phase blocking ELISA (LPBE) using different antigen concentrations, and reagents prepared by The World Reference Laboratory (WRL) for FMD. It was evident that, not only the concentration of antigen but the ratio of antigen to antibodies as well as antibody nature (avidity) are the most important determining factors in detection of serum blocking activity.

 

25

Lowest percentage inhibition (PI) values for a given serum were detected when antigen and antibodies were expected to be about equal, and highest values were detected when antigen was in excess. Poor correlation between positive reference antisera (experimental infection) and positive test sera (natural exposure) was particularly evident when relatively low concentrations of antigen were used.  It is concluded that increase in antigen concentration increases the detected specific blocking activity as excess-antigen immune complexes are more efficiently blocked than equivalent-antigen immune complexes, and high avidity antibodies, in weak positive sera, react preferentially. Antigen concentrations that represented less than two fold increase of the pre-estimated dose and that gave OD values well below the predefined limit of 1.9, increased sensitivity of the assay to desired levels.

 
 
   
 
 

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