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ãáÎÕ ÇáÈÍË
áÊÍÏíÏ ÇáÌÑÚÉ ÇáÇßËÑ ãáÇÆãÉ ãä ÇáãÓÊÖÏ áÇÎÊÈÇÑ ÇáÊáÇÒä ÇáãäÇÚí ÇáãÊÕá ÈÇáÅäÒíã Ðì ÇáØæÑ ÇáãÇÆÚ ÇáãÍÕÑ (Liquid-Phase Blocking ELISA)  áãÞÇíÓÉ ÇáãÓÍ (Screening Assay) áãÑÖ ÇáÍãí ÇáÞáÇÚíå‘ ÊãÊ ãÚÇíÑÉ ÚíäÇÊ ãÕáíÉ ááÃÖÏÇÏ ÇáäæÚíÉ ááãÓÊÖÏ "SAT1" ÈÇÓÊÚãÇá ÌÑÚÇÊ ãÎÊáÝÉ ãä ÇáãÓÊÖÏ ÇáãÐßæÑ ÈÏÁÇ" ÈÇáÌÑÚÉ ÇáÊí ÊÚØí ãÚÏá ßËÇÝÉ ÖæÆíÉ(OD value) ÈãÞÏÇÑ1.5. æÌÏ Ãä ÇáÌÑÚå ÇáÇßËÑ ãáÇÆãå ÊÍÏË ãäÍäíÇÊ ãÚÇíÑÉ ããÇËáå áãÎÊáÝ ãÌãæÚÇÊ ÇáãÕá ÇáãæÌÈå (ÚÇáíÉ-ãÊæÓØÉ-ÖÚíÝÉ).
ÚäÏ ÇÓÊÚãÇá ÊÑßíÒ ãäÇÓÈ ãä ÇáãÓÊÖÏ  íßæä ãä Çáããßä ÊÓÌíá ÃÚáí äÔÇØ ÅÍÕÇÑí (Maximum plateau height of blocking activity) (ÃÞÑÈ ãÇ íßæä Çáí 100%) ßãÇ Ãä ÇáÚáÇÞÉ ÇáÊáÇÒãíÉ (correlation) Èíä ÊÎÝíÝÇÊ ÇáÃÖÏÇÏ ÇáäæÚíÉ Ýí ÇáÚíäÉ ÇáãÕáíÉ æ ÞáÉ ÇáäÔÇØ ÇáÃÍÕÇÑì Êßæä Ýí ÃÍÓä ÍÇáÇÊåÇ.
ÚäÏ ÇÓÊÚãÇá ÊÑßíÒ ÃÞá íßæä ÃÚáí äÔÇØ ÅÍÕÇÑí  íãßä ÊÓÌíáå ÃÞá ããÇ íÌÈ Úáíå Ãä íßæä (ÃÞÑÈ Åáí% 70 æ %80) ßãÇ Ãä ÇáäÔÇØ ÇáÃÍÕÇÑí  ÇáãÓÌá íäÎÝÖ ÈãÚÏáÇÊ ãÑÊÝÚÉ áÇ ÊÊäÇÓÈ æ ÊÎÝíÝÇÊ ÇáÃÖÏÇÏ ÇáäæÚíÉ Ýí ÇáÚíäÉ ÇáãÕáíÉ . Ýí åÐå ÇáÍÇáÉ íãßä Ãä Êßæä ÇáãÚÇíÑÉ ÇÞá ÍÓÇÓíÉ(less sensitive) æ ÃíÖÇ" ÐÇÊ äæÚíå ÃÞá (less- specific).
  ÚäÏ ÇÓÊÚãÇá ÊÑÇßíÒ ÚÇáíÉ ãä ÇáãÓÊÖÏ íßæä ÇáäÔÇØ ÇáÅÍÕÇÑí ÃÞá¡  ÎÇÕÉ ãÚ ÇáÚíäÇÊ ÇáãÕáíÉ ÐÇÊ ÇáÊÑßíÒ ÇáãäÎÝÖ æ ÇáãÊæÓØ ãä ÇáÃÖÏÇÏ ÇáäæÚíÉ.


Summary
        To determine the optimal antigen dose for anti-type SAT1 virus antibody detection liquid-phase blocking ELISA screening assay of FMD, a panel of local and control sera were titrated using different concentrations of  the antigen, starting from a concentration that gives an OD value of 1.5.The optimal antigen concentration was found to produce parrallel titration curves for the different positive serum groups (strong, medium and weak). At such concentration, the screening assay correlates well with the titration assay. The sera that showed the highest positive percentage inhibition (PI) value in screening assay showed the highest titre and vice versa. Maximum plateau height of blocking activity (near 100%) was produced and good correlation between serum dilutions and the decrease in positive blocking activity did frequently exist.
      
At low antigen concentrations, no correlation was observed between screening and titration assay; sera that showed similar positive PIs in screening assay showed different titres or vice versa. Comparativley low plateau height (70% and 80%), poor correlation and/or sharp decrease in detected positive blocking activity following serum dilution, marked these lower concentration. The assay might be both insensitive and less specific. High antigen doses were marked by a decrease in the detected blocking activity, particularly, of weak and medium positive sera (insensitivity).

 

 
 
   
 
 

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