Sixty water samples were collected from ten poultry farms in Khartoum State during May to September 2014. The samples were collected from tap water, tanks and drinkers in sterile sample bottles. Nine genera of moulds were isolated: Aspergillus, Penicillium, Mucor, Curvularia, Cladosporium, Rhizomucor, Aureobasidium, Alternaria and Exserohilum were isolated from tap water, tanks, and drinkers. Cryptococcus albidus and Cr. Laurentii from tanks and Candida rugosa from drinkers.