Polymerase chain reaction (PCR) was conducted on DNA samples from chicken infected with fowl pox, pigeon and canary pox viruses. Two set of primers were used, fowl pox major envelope antigen gene primer (FPVORF 108) for the detection of fowl, pigeon and canary pox viruses, while REV LTR primer was used for the detection of the REV provirus in local isolates of fowl, pigeon and canary pox virus. Pigeon and canary pox viruses isolates with FPV (ORF 108) gave positive results with a product size of 1,222 bp. Fowl pox field isolates with REV LTR primers, gave positive result with product size of 900 bp, while canary and pigeon pox virus were negative. This study shows that PCR is most suitable for the diagnosis of fowl pox infection for its simplicity and relative rapidity (1-2 days) when clinical signs, postmortem lesions and histopathological findings are not clear. In addition, virus isolation is laborious, time consuming and would take 7-14 days. To the best of our Knowledge, this is the first report of integration on RE provirus in fowl pox virus field isolates in the Sudan.