In the present study, 808 donkeys in Khartoum State were investigated for wounds bacterial infections. The investigation included isolation and identification of the contaminating bacteria, and determination of their sensitivity to different antibacterial drugs. The information collected included, age, sex, type of work, season, duration of work, site of wound and type of treatment. Wounds were detected in 249 (30.8%) of the investigated animals. Twenty-five bacterial species were isolated. The isolates were identified as Rhodococcus equi (31.68%), Staphylococcus spp. (31.06%), Micrococcus spp. (12.11%), Corynebacterium sp. (5.59%), Actinomyces spp. (5.59%), Bacillus sp (4.96%), Streptococcus spp. (2.8%), Pasteurella sp. (1.86%), Burkholderia sp. (1.24%), Neisseria sp. (1.24%), Acinetobacter sp. (0.93%), Fusobacterium sp. (0.93%), Listeria sp. (0.62%), Brucella sp. (0.62%), Actinobacills sp. (0.62%), Yersinia sp. (0.62%), Flavobacterium sp. (0.31%) and Mannheimia sp. (0.31%). Fifteen bacterial species were tested for their sensitivity to six antibacterial drugs. Brucella abortus was resistant to the all tested antibiotics. Rhodococcus equi was resistant to ampicillin and moderately sensitive to tetracycline. The collected data revealed that 45.9% of the wounded donkeys were less than 8-year-old and 35.54% of them were wounded during summer. Most of the wounds (71.55%) were present on the back of the animal, 14.22% at abdominal region and 11.5% on the legs. There is direct relation between the site of wound and type of work, and most of the wounded animals were either treated with traditional methods or drugs. The study revealed that 59.44% of infected animals suffered from primary skin diseases; 35.09% of them suffered from subcutaneous abscesses whereas 20.48% of infected animals suffered from secondary skin diseases of which 14.60% had fistulous withers.