Fasciolosis is one of the major veterinary problems in the world that reduces the animal production. In the present study, a total of 3600 faecal samples (1800 from each of buffaloes and cattle) was collected on a random basis from different areas of Bahawalpur between February and October, 2013. During sampling the animals body condition and their deworming history were recorded. An overall prevalence of fasciolosis in buffaloes and cattle amounted to 15.8% and 14.2%, respectively. Furthermore, in both buffaloes and cattle it was found that poor body conditions (43.9% and 48.8%, respectively) and no deworming (25.9% and 24.6%, respectively) favoured animal susceptibility to infection. Fasciola gigantica was the major infection in both buffaloes and cattle (11% and 10.1%, respectively) as compared to that of Fasciola hepatica (buffaloes 3.61% and cattle 2.9%).