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Polymerase chain reaction (PCR) was used for detection of trypanosomes in blood on FTA cards. Ninety-seven blood samples collected from infected cattle from different areas of Sudan were tested. The PCR was carried out according to the protocol of purification of invitrogen Purelink genomic DNA kits, using three sets of primers. The first set included species specific primers to Trypanosoma (Nannomonas) congolense subsp Savannah and Kilifi, T. (Trypanozoon) brucei and T. (Dunonella) vivax. The second set contained ITS primers which were used as a single test and the third set consisted of ITS primers that were used in a nested PCR. The results of the PCR indicate that in addition to the presence of T. vivax and T. brucei, the only T. congolense taxonomic group identified in Sudan belongs to the Savannah. No infection attributable to T. congolense Kilifi was detected. This work confirms the existence of mixed infections in the field, which cannot be detected easily by the conventional parasitological methods.

 
 
   
 
 

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