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Summary This study was carried out in Khartoum State to determine contamination of poultry rations with fungi including Aspergillus flavus and measurement of aflatoxin concentration using ELISA and fluorometry techniques. A total of 160 poultry rations (80 samples of layer and broiler rations each) were collected from poultry farms. Poultry rations were cultured on Sabouraud’s Dextrose Agar, supplemented with chloramphenicol and observed periodically for development of fungal growth. Fungal colonies were identified on the basis of their colonial morphology and cultural characteristics. Seventy samples (87.5%) of layer feed and 62 samples (77.5%) from broiler ration showed contamination with fungi. Analysis of aflatoxin in contaminated rations revealed that 25% of the rations had aflatoxin concentration above the permissible level (20ppb) according to FAO and FDA regulations. The study also, revealed that fluorometric analysis was more sensitive than ELISA and that broiler feed was more contaminated compared to layer feed.

 
 
   
 
 

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