This study was carried out in Khartoum State to determine contamination of
poultry rations with fungi including Aspergillus flavus and measurement of
aflatoxin concentration using ELISA and fluorometry techniques. A total of 160
poultry rations (80 samples of layer and broiler rations each) were collected from
poultry farms. Poultry rations were cultured on Sabouraudâ€™s Dextrose Agar,
supplemented with chloramphenicol and observed periodically for development
of fungal growth. Fungal colonies were identified on the basis of their colonial
morphology and cultural characteristics. Seventy samples (87.5%) of layer feed
and 62 samples (77.5%) from broiler ration showed contamination with fungi.
Analysis of aflatoxin in contaminated rations revealed that 25% of the rations
had aflatoxin concentration above the permissible level (20ppb) according to
FAO and FDA regulations. The study also, revealed that fluorometric analysis
was more sensitive than ELISA and that broiler feed was more contaminated
compared to layer feed.