This study was performed to determine the prevalence of type specific neutralizing antibodies to African horse sickness virus (AHSV) in equidae and some other animal species in Khartoum State, Sudan. Antibodies (Abs) to AHSV were detected in horses, donkeys, goats, cattle and Dorcas gazelle (Order: Artiodactyla). Antibodies to all AHSV serotypes were detected except AHSV-8 because its antigen was not available during this study. The detection of antibodies to AHSV in goats, cattle and Dorcas gazelle points out to the possibility that, these animals might have been exposed to a subclinical infection. The detection of Abs to AHSV in Dorcas gazelle was the first to be reported in the Sudan; consequently, further studies are required to clarify this finding.