This study was carried out to determine the prevalence of Anaplasma marginale among cattle in South Kordofan State. A total of 575 blood smears was collected from different eco-types of indigenous (zebu) cattle including Baggara, Kenana, and Umbararow in addition to cross breed cattle (zebu x friesian) of both sex and different age groups. Microscopic examination revealed low parasitaemia detected in only 8 % of cattle examined during this study. Based on microscopic examination, the highest prevalence was reported in Dilling locality (16%). Statistically, there was no significant difference between prevalence rates in the different localities and among different types of indigenous cattle (P ³ 0.05). However, significant difference (P£ 0.05) was found among different age groups. A total of 208 selected serum samples from Dilling and Kadugli localities was subjected to ELISA test to assess antibodies against Anaplasma marginale. The overall seroprevalence rate of A. marginale was found to be 66 %. The highest seroprevalence was reported in Dilling Locality (89%). Unlike the results of the blood smears, ELISA test showed significant correlation (P £ 0.5) between the occurrence of A. marginale and both age and two eco-types of indigenous cattle with very high percentages of antibodies. This study reveals that antibodies to A. marginale infections are widely distributed in South Kordofan State.