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Summary Five hundred and fourty dead-in-shell chick embryos were obtained from four hatcheries of big poultry farms in Khartoum state. From these, 212 bacterial isolates were isolated consisted of 57 (26.89%) Gram-positive organisms and 155 (73.11%) Gram-negative organisms. The isolated Gram-positive bacterial genera were Staphylococcus (19.81%), Corynebacterium (2.36%), Bacillus (2.83%), Streptococcus (0.94%) and Arcanobacterium (0.94%). The Gram-negative bacterial genera were Proteus (11.79%), Escherichia (10.84%), Klebsiella (8.01%), Pseudomonas (6.60%), Citrobacter (6.13%), Chromobacterium (5.7%), Yersinia (3.77%), Aeromonas (3.30%), Salmonella (2.35%), Alcaligenes (2.35%), Flavobacterium (2.35%), Morganella (2.35%), Serratia (1.88%), Enterobacter (1.41%), Erwinia (0.94%), Janthinobacterium (0.94%), Plesiomonas (0.94%), Providencia (0.94%), and Bordetella (0.47%). The organisms which were isolated from all farms were Staphylococci, Salmonella, Escherichia coli, Klebsiella, Proteuse, and Yersinia enterocolitica.

 
 
   
 
 

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