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Summary
Polymerase Chain Reaction (PCR) and Random Amplification Polymorphic DNA
(RAPD) were used for identification of six isolates, three of each Marnheimia, haemolytica and
Pasteurella multocida were isolated from pneumonic lungs of sheep and collected from El-
Damazin slaughterhouse, Blue Nile State, the Sudan. PCR was performed in a total reaction
volume of 25μl and the random amplification Polymorphic DNA in volume of 50μl . All test
isolates of M. haemolytica P. multocida were identified biochemically and confirmed by PCR
and RAPD analysis. PCR profiles for M. haemolytica and P. multocida were found
corresponding to the anticipated size of 200 bp and 237 bp, respectively while RAPD analysis
generated four and five bands for M.haemolytica and P.multocida respectively. PCR Technique
was found useful, specific and time saving for identification of P.multocida, and M. haemolytica.
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