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Summary The effect of temperature and relative humidity (Rh) regimen on the biological cycle of the immature stages of Amblyomma lepidum was studied under laboratory conditions. The moulting process was studied at 27°C, 35°C and 40°C, with ultimate five sets of humidity (97.8%, 85%, 75.5%, 51.2% and 32.4%). At 27 °C and 97.8% Rh the mean per cent moult was 93.4%, while at 35°C under the same humidity, it was 100%. At 85% Rh, the moult percentage declined to 31.8% and 43.6% at 27°C and 35°C, respectively. At the three lower levels of Rh and temperature of 27°C and 35°C, no larvae were moulted. Nymphs at both 27°C and 35°C, succeeded to moult even at the lower end of Rh, reaching a mean of 99.4%. However, at 40°C regardless of the Rh, both larvae and nymphs died before the commencement of moulting.

 
 
   
 
 

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