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Summary The DNA profile of four local strains of Clostridium chauvoei and the vaccinal strain Kad were compared with each others and with that of a reference strain by polymerase chain reaction (PCR). The pathogenecity of the four isolates and the strain Kad was compared . Detection by PCR using specific primers for C. chauvoei have revealed that the four local isolates and strain Kad were identical and confirmed that their members has to C. chauvoei. Inoculation of guinea pig with each isolate, showed typical blackleg lesions such as swelling and haemorrhage. Muscles were black and dark in colour with gas and rancid odour. Histopathology showed inflammatory changes that include haemorrhage, necrosis and gas bubbles which are typical blackleg lesions.

 
 
   
 
 

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