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Summary The Saposin -like recombinant protein from Fasciola gigantica was used as antigen in ELISA for the detection of F. gigantica antibodies in naturally infected cattle in the Sudan. When compared with the results of Excecretory Secretory (ES) antigens and Sedimentation tests, the SAP-2 antigen gave a sensitivity of 85% (111/130) by the ELISA test, and specificity of 86%. Using the ES antigen, the sensitivity was 80% (104/130) and the specificity was 53% (50/94). On the other hand, the sensitivity and specificity of the Sedimentation test was 75% and 100%, respectively. The FgrSAP-2 proved to be more suitable and useful tool for the diagnosis of fasciolosis than the FgES and Sedimentation tests.

 
 
   
 
 

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