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ãáÎÕ ÇáÈÍË
                  Ýì åÐå ÇáÏÑÇÓÉ ÚÒáÊ æÍÏÏÊ åæíÉ ÇáãÝØæÑÉ ãÚÝäÉ ÇáÏÌÇÌ (Mycoplasma gallisepticum) ãä ãÓÍÇÊ ãÍ ÃÌäÉ ÚãÑ ÓÈÚÉ ÃíÇã æåæÇÁ ÍÙíÑÉ ÏÌÇÌ ÃãåÇÊ ÈãÏíäÉ ÇáÎÑØæã ÓÈÞ ÊØÚíãå ÖÏ åÐå ÇáãÝØæÑÉ. ÇÓÊÎÏãÊ ÇáæÓÇÆØ ÇáÛÐÇÆíÉ ÇáãäÇÓÈÉ áÒÑÇÚÉ ÇáãÝØæÑÇÊ (Mycoplasma Broth and Agar Media) æÃÌÑì ÇáÊÝÇÚá Úáí ÇáãÊÓáÓá ááÈíæáíãÑíÒ (PCR) ááãÚÒæáÇÊ æÇáÐí ÃËÈÊ ÙåæÑ ÇáÒæÌ ÇáÞÇÚÏí 140 ããÇ íÄßÏ æÌæÏ ÇáãÝØæÑÉ ãÚÝäÉ ÇáÏÌÇÌ. æÌÏ Ãä ÇáÊÝÇÚá ÇáãÊÓáÓá ááÈæáíãÑíÒ íÊãíÒ ÈÇáÍÓÇÓíÉ æÇáäæÚíÉ ÇáÊì ÊÄåáå ááÚÈ ÏæÑ Ýí ÏÑÇÓÉ æÈÇÆíÇÊ ÇáãÝØæÑÇÊ æÇáÓíØÑÉ Úáí ÊáæíËåÇ áãÒÇÑÚ ÇáÏæÇÌä.  

Summary
In this study, Mycoplasma gallisepticum was isolated and identified from yolk and air of an open vaccinated poultry breeder farm in Khartoum state, Sudan. Swabs from 7-day-old embryonated yolk as well as from air inside the poultry houses of this farm were collected. They were cultured into Mycoplasma Broth and onto Agar media. The isolates were then subjected to PCR technique. Positive bands of 140 bp were seen, using PCR kit for M. gallisepticum, which confirmed the presence of the organism. The PCR was specific and sensitive to play a role in the epizootiology and control of farm contamination  with mycolplasmas.

 

 
 
   
 
 

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