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ãáÎÕ ÇáÈÍË ÏÑÓÊ ÇáÅÓÊÌÇÈÇÊ ÇáãäÇÚíÉ áßÊÇßíÊ ÇáÈíÇÖ ÈÇááÞÇÍÇÊ ÇáÍíÉ ÇáÔÇÆÚÉ ÇáÅÓÊÚãÇá ÖÏ ãÑÖ ÇáäíæßÇÓá Ýì ÇáÓæÏÇä. .(A,B,C and D) ( ÇáãÊÛíÑÇÊ ÇáÊì ÊãÊ ÏÑÇÓ滂 ªì äæÚ ÚÊÑÉ ÇááÞÇÍ æ ØÑÞ ÅÚØÇÆå. Êã ÅÎÊÈÇÑ ÃÑÈÚÉ áÞÇÍÇÊ (Ã¡È¡Ì æÏ ÃÚØì ßá áÞÇÍ ÈËáÇËÉ ØÑÞ: ÇáÑÔ ÇáÑÐÇÐì¡ÇáÊäÞíØ Ýì ÇáÃäÝ æ Úä ØÑíÞ ãÇÁ ÇáÔÑÈ. Êã ÞíÇÓ ÚíÇÑ ÃÖÏÇÏ ÝíÑæÓ ÇáãÑÖ Ýì ãÌãæÚÇÊ ÇáßÊÇßíÊ ÇáãáÞÍÉ æ ÇáãÌãæÚÉ ÇáÛíÑ ãáÞÍÉ (ãÌãæÚÉ ÇáãÑÇÞÈÉ) .(ELISA) æ ãÞÇíÓÉ ÇáÃÔÊÑÇÈ ÇáãäÇÚì ÇáãÑÊÈØ ÈÇáÅäÒíã (HI) ÈÅÎÊÈÇÑ ÊËÈíØ ÇáÊÑÇÕ ÇáÏãæì ÃÙªÑ ÊËÈíØ ÇáÊÑÇÕ ÇáÏãæì æÌæÏ ÅÎÊáÇÝÇÊ Ýì ÇáÅÓÊÌÇÈÉ ÇáãäÇÚíÉ äÊíÌÉ ááÅÎÊáÇÝ Ýì ÚÊÑÉ ÇááÞÇÍ. äÊÇÆÌ ãÞÇíÓÉ ÇáÅÔÊÑÇÈ ÇáãäÇÚì ÇáãÑÊÈØ ÈÇáÅäÒíã ÃßÏÊ äÊÇÆÌ ÊËÈíØ ÇáÊÑÇÕ ÇáÏãæì ßãÇ ÃÙªÑÊ Ãä äÓÈÉ ÇáÊÌÇäÓ Ýì ÚíÇÑ ÇáÃÖÏÇÏ ßÇäÊ ÇáÃÞá Ýì ÈÛÖ ÇáäÙÑ Úä ØÑíÞÉ ÃÚØÇÁ ÇááÞÇÍ. (A) ÇáãÌãæÚÇÊ ÇáÊì áÞÍÊ ÈÇááÞÇÍ Ã Êã ÅÌÑÇÁ ÅÎÊÈÇÑ ãÞÇæãÉ áãÌãæÚÇÊ ÇáßÊÇßíÊ ÈÅÓÊÎÏÇã ÚÊÑÉ ÍÞáíÉ ÖÇÑíÉ áãÑÖ ÇáäíæßÇÓá. áã ÊÙªÑ Ãì ÚáÇãÇÊ ÓÑíÑíÉ ááãÑÖ Ãæ äÝæÞ Ýì ÌãíÚ ÇáãÌãæÚÇÊ ÇáãáÞÍÉ ØæÇá ÝÊÑÉ ÇáÅÎÊÈÇÑ ÈíäãÇ ÈÏÃÊ ÊÙªÑ ÃÚÑÇÖ ÓÑíÑíÉ (Îãæá¡ÃäÊÝÇÔ ÑíÔ¡ÅÓªÇá ÃÎÖÑ æ ÕÚæÈÉ ÊäÝÓ) Ýì ãÌãæÚÉ ÇáãÑÇÞÈÉ ÛíÑ ÇáãáÞÍÉ ÅÈÊÏÇÁð ãä Çáíæã ÇáÑÇÈÚ ááÅÎÊÈÇÑ æ ÞÏ äÝÞÊ ÌãíÚ ÇáßÊÇßíÊ ÈÍáæá Çáíæã ÇáÊÇÓÚ ááÅÎÊÈÇÑ. Summary This study was conducted to evaluate the immune responses of layer chicks to the live Newcastle Disease (ND) vaccines commonly used in the Sudan. Variables studied were vaccine strain and vaccination route. Four commercial live ND vaccines (A, B, C and D) were administered via three different routes (Spray, Intra-nasal and Drinking water). Anti ND virus (NDV) antibodies titres were determined by Haemagglutination Inhibition test (HI) and Enzyme Linked Immuno-Sorbent Assay (ELISA) where HI results showed variation in immune response due to vaccine strain. ELISA results confirmed the HI results and revealed that antibodies homogeneity was best in vaccine A by all routes. A challenge test using virulent field isolate was carried out. No clinical signs were observed up to day 10 in all the vaccinated groups. The unvaccinated group manifested clinical signs (dullness, ruffled feathers, respiratory distress and greenish diarrhoea) beginning on day 4 post challenge and all chicks were died by day 9 post challenge.

 
 
   
 
 

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