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ãáÎÕ ÇáÈÍË
         Êã ÊÕäíÝ 48 ãÚÒæáÉ áÈßÊÑíÇ ÇáÚäÞæÏíÉ ÇáãÊæÓØÉ (Staphylococcus intermedius) ãä ÇáßáÇÈ æÇáÞØØ ÈæÇÓØÉ ËáÇË ØÑÞ ááÊÕäíÝ ÇáÑÍáÇä ÇáßåÑÈÇÆí Úáí ãÇÏÉ ÚÏíÏ ÃßÑíá ÃãÇíÏ. (Polyacrylamide) æÇáÊÎØíØ ÇáãäÇÚí(Immunoblotting) æ ÇáãÞÇíÓÉ ÇáãäÇÚíÉ ÇáãÑÊÈØÉ ÈÇáÅäÒíã (ELISA) . Êã ÇáÊÚÑÝ Úáì 16 ãÌãæÚÉ ÈØÑíÞÉ ÇáÑÍáÇä ÇáßåÑÈÇÆí æ 12 ãÌãæÚÉ ÈØÑíÞÉ ÇáÊÎØíØ ÇáãäÇÚí. ÎáÕÊ ÇáÏÑÇÓÉ Åáí Ãä ÇÓÊÚãÇá åÇÊíä ÇáØÑíÞÊíä íÓÇÚÏ Ýí ÊÕäíÝ ÇáÈßÊÑíÇ ÇáÚäÞæÏíÉ ÇáãÊæÓØÉ.  ÈíäãÇ ÇáãÞÇíÓÉ ÇáãäÇÚíÉ ÇáãÑÊÈØÉ ÈÇáÎãíÑÉ ááÈÑæÊíä à ÊÓÇÚÏ ÝÞØ Úáí ÅËÈÇÊ æÌæÏå æ ÇáÊÚÑÝ Úáíå Ýí ÇáÚíäÇÊ æ áßä áÇ íãßä ÇÓÊÎÏÇãåÇ ááÊÕäíÝ.

Summary
Forty-eight Staphylococcus intermedius isolates from dogs and cats were characterized using sodium dodecyle sulphate Polyacrylamide Gel electrophoresis (SDS-PAGE) of whole cell proteins, immunoblotting and protein A assay by ELISA. Sixteen and 12 profiles were recognized using SDS-PAGE and immunoblotting, respectively. It is concluded that SDS-PAGE of whole cell proteins and immunoblotting could be used for the characterization and typing of S. intermedius isolates. However, protein A assay, could be used for the detection of S. intermedius isolates in samples but can not be used to differentiate between different isolates.


 

 
 
   
 
 

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